Oral Presentation RACI Biomolecular Division Conference 2013

Development of levofloxacin releasing  intravitreal implants for the treatment of ocular disease (#35)

Andrew C Donohue 1 2 , Frank Wang 2 , Richard Prankerd 2 , Russell Tait 1 2 , Sarah Ng 1 2 , Asha D'Souza 1 2 , Hong Zhang 3 , Emily Hart 4 , Roy Robins-Browne 4 , Louise Adams 4
  1. PolyActiva Pty. Ltd., Melbourne, Victoria, Australia
  2. Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, Victoria, Australia
  3. Centre for Eye Research Australia, The University of Melbourne, Melbourne, Victoria, Australia
  4. Microbiology and Immunology, The University of Melbourne, Melbourne, Victoria, Australia

PolyActiva is developing ocular implants that enable slow release, site specific drug delivery directly to the eye, from an implant that erodes completely. They are produced from a proprietary drug-polymer conjugate technology.  Key attributes include:

·         Higher drug loadings per mass of polymer than currently available.

·         Controlled and selective drug release with drug pendent to the polymer backbone.

·         Complete polymer bioerosion leaving no residue and producing safe by-products

·         A modular nature that enables properties to be tuned to meet requirements

An intravitreal implant that delivers the antibiotic levofloxacin (LVX) for the post-surgical prophylaxis and treatment of bacterial endophthalmitis (a catastrophic inflammatory reaction to eye infection) has been developed.  LVX was covalently attached to a monomer unit via a selectively labile linker and polymerised with suitable co-monomers to give a final polymer implant with the desired characteristics. Performance was assessed by analysis of in vitro release kinetics and evaluation of pharmacokinetics and efficacy in an animal model.

Results showed:

·         At the high drug loadings achieved (ca. 50%) the drug’s physicochemical properties played a significant  role in the polymer’s attributes, including drug release rates.

·         Zero-order release of LVX over at least 30 days.

·         Drug release is a function of implant mass

·         In vitro release of levofloxacin (5-40 mg/day) at therapeutic levels over an extended period (up to 120 days).

·         LVX concentrations in vitreous matched steady-state levels predicted from pharmacokinetic calculations based on the in vitro release rate, and were well above the MIC for S. aureus for at least a 10-day treatment period.

·         Infection is cleared or prevented in a validated rabbit endophthalmitis model from administration of a single implant.

A second ocular implant programme for the delivery of an agent for the treatment of glaucoma is underway.